Genomic data

Chen et al. PLoS Genetics 13:e1006863, 2017.
RNA tiling array data are available from the NCBI Gene Expression Omnibus, accession number GSE100283

True et al. JBC 291:15307-15319, 2016.
ChIP-seq, MNase-seq and RNA tiling data are available from the NCBI Gene Expression Omnibus, SuperSeries assession number GSE80235

Hyland et al. Genes & Dev. 25:1306-1319, 2011.
RNA tiling array data are available from the NCBI Gene Expression Omnibus, accession number GSE13889

Poorey et al. Genome Res. 20:1679-1688, 2010.
RNA tiling and ChIP-on-chip data are available from the NCBI Gene Expression Omnibus, accession number GSE18283
Download processed RNA and ChIP-chip data files here.

Wade et al. EMBO J. 28:2919–2931, 2009.
all microarray gene expression data reported in this study are available from the NCBI Gene Expression Omnibus (GEO), accession number GSE16799

Sprouse et al. JBC 284:4525-4535, 2009.
bypass TBP microarray data are available from the NCBI Gene Expression
Omnibus, accession number GSE12371

Miyake et al. JBC 279: 34865-34872, 2004.
view list of Abf1-regulated genes with links to SGD

Dasgupta et al. JBC 279:26830-26838, 2004.
san1 microarray data are available from YFGdb

Dasgupta et al. PNAS 99:2666-2671, 2002.
browse or download mot1 microarray data

Microarray Data Parser: here

Methods

Affinity purification of Mot1
ATPase assay
ChIP (standard assay)
ChIP-chip protocol
ChIP-seq
FeBABE cleavage protocol
Gel shift assay
Immobilized template assay
In vitro NER assay
Northern blot protocol
pCORE modification protocol
Plasmid mini prep
Primers for yeast genome modification
Preparation of Py antibody-coupled beads
Quantitation of RNA by real time PCR
Yeast whole cell extract prep (galactose-induced cells)
Yeast genomic DNA prep
Yeast genomic DNA prep (10 min)
Yeast total RNA prep
Yeast transformation for integration (tagging, KO, pCORE, etc)
Yeast transformation